About The Book
Column chromatography is a commonly used purification technique in labs across the world. Done right it can simply and quickly isolate desired compounds from a mixture. But like many aspects of practical chemistry, the quick and efficient setting up and running of a column is something that can take years to master. Here we provide some tips and tricks for running a column, troubleshooting for some problems commonly encountered during column chromatography, and some “quick and dirty” alternatives to a full column. We have previously looked at how to pack a column and how to choose the necessary equipment and solvents. Once you have set this up, you will be ready to load and run your column. Loading the column involves placing the sample directly onto the top of the column and allowing it to absorb onto the silica.
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